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In the automated Sanger reaction, primers are used that are labeled with four different colored fluorescent tags.

PCR reactions, in the presence of the different dideoxy nucleotides, are performed as described above.

March 2010 - July 2010Rumors ran wild during the 2010 season of "Dancing with the Stars" when sportscaster Erin Andrews and pro Maksim Chmerkovskiy were paired together.In the late 1970's, two DNA sequencing techniques for longer DNA molecules were invented.These were the Sanger (or dideoxy) method and the Maxam-Gilbert (chemical cleavage) method.The advent of PCR opened up many doors in genetic research, including a means of DNA analysis and identification of different genes based on their DNA sequences.DNA sequencing is also dependent on our ability to use gel electrophoresis to separate strands of DNA that differ in size by as little as one base pair.Each PCR reaction will end up containing a mixture of different lengths of DNA strands, all ending with the nucleotide that was dideoxy labeled for that reaction.


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